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    Nov 20, 2025
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    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 1 Summative Assessment: Health Information Management Using and Understanding Public Health Data Paper

    Due Date: 12/2/25, 12:59 AM (CST)

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    Nov 20, 2025
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    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 1 – Course Capstone Project – Preparation and Statement of Need

    Due Date: 12/2/25, 12:59 AM (CST)

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    Nov 20, 2025
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    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 1 Discussion – Data Collection (initial response due Saturday)

    Due Date: 12/2/25, 12:59 AM (CST)

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    Nov 20, 2025
    11:27 PM

    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 2 – Course Capstone Project – Annotated Bibliography

    Due Date: 12/9/25, 12:59 AM (CST)

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    Nov 20, 2025
    11:27 PM

    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 2 Discussion – Disease Prevention [due Thurs]

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    ## MPH/530: Epidemiology Concepts And Public Health Diseases

    OpenCourse status Open

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    #### [Course Questions](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/discussion/_52313045_1?view=discussions&courseId=_547585_1)

    Have a question about this course? Ask here! Your faculty member will monitor this area. If you know the answer, feel free to help your peers by posting a reply.

    #### Welcome to the Course and Getting Started

    Start with this folder to meet your faculty member and peers. Review the course guide, syllabus, and grading rubrics for an overview of this course.

    #### Wk 1 – Community and Population Health Data

    [Wk 1 Overview](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/edit/document/_52313060_1?courseId=_547585_1&view=content&state=view) [Wk 1 Discussion – Data Collection (initial response due Saturday)](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/discussion/_52313047_1?view=discussions&courseId=_547585_1)

    Due date: 12/2/25, 12:59 AM (CST)

    Post a total of 3 substantive responses over 2 separate days for full participation. This includes your initial post and 2 replies to classmates or your faculty member. Due Saturday because of Thanksgiving To prepare for this week’s discussions, begin by selecting and reading 1 article from the University Library. Then, respond to the following questions in a minimum of 175 words: What data collection method(s) did the researcher use? Why did they choose this method(s)? Were they appropriate? Why or why not? Were the methods effective or ineffective for gathering data and proposing interventions for the targeted community? Why or why not? What rationale, examples, and comparisons would you use to defend your perspectives? What other …

    [Wk 1 Summative Assessment: Health Information Management Using and Understanding Public Health Data Paper](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/assessment/_52313062_1/overview?courseId=_547585_1)

    Due date: 12/2/25, 12:59 AM (CST)

    This assessment measures your ability to develop data collection and information organization and retrieval plans.

    [Wk 1 – Course Capstone Project – Preparation and Statement of Need](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/assessment/_52313063_1/overview?courseId=_547585_1)

    Due date: 12/2/25, 12:59 AM (CST)

    Wk 1 Learning Activities

    [Syringe Exchange Programs! A great harm reduction tool (video by Bob)](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/edit/document/_52322287_1?courseId=_547585_1&view=content&state=view)

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    #### Wk 2 – Disease Prevention and Program Implementation

    #### Wk 3 – Social Justice and Health Equity in Epidemiology

    #### Wk 4 – Examining Epidemiology Research Design

    #### Wk 5 – Evaluating Epidemiology Research Methods

    #### Wk 6 – Applying Epidemiology Research Methods

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    ### Course Faculty

    – ![Bob Vella](https://www.sweetstudy.com/questions/health-information-20951863)

    Bob Vella

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    MPH/530: Epidemiology Concepts And Public Health Diseases

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    ## Wk 1 Summative Assessment: Health Information Management Using and Understanding Public Health Data Paper

    ### Assignment Instructions

    Research data from a provided case study to understand how it impacts the population.

    Write a 700- to 1,400-word paper in which you will include the following:

    1. Select 1 of the following case studies. Then, conduct additional research to gain an understanding of the chosen public health issue.
    2. [Notes from the Field: Multistate Outbreak of Escherichia coli O157:H7 Infections Linked to a National Fast-Food Chain — United States, 2022](https://www.cdc.gov/mmwr/volumes/72/wr/mm7226a6.htm)
    3. [Notes from the Field: Multistate, Multiserotype Outbreak of Salmonella Infections Linked to Cashew Brie — United States, 2021](https://www.cdc.gov/mmwr/volumes/72/wr/mm7221a4.htm)
    4. [Multistate Outbreak of Salmonella Thompson Infections Linked to Seafood Exposure — United States, 2021](https://www.cdc.gov/mmwr/volumes/72/wr/mm7219a2.htm)
    5. [Outbreaks Associated with Untreated Recreational Water — California, Maine, and Minnesota, 2018–2019](https://www.cdc.gov/mmwr/volumes/69/wr/mm6925a3.htm)
    6. [Notes From the Field: Campylobacteriosis Outbreak Associated with Consumption of Raw Water — Montana, 2022](https://www.cdc.gov/mmwr/volumes/72/wr/mm7215a6.htm)
    7. Identify data from within the selected case study. Summarize the demographic and clinical information of the data.
    8. Enter data into an Excel file and produce a graphical visual display of the data (e.g., chart or graph of demographic data, histogram, pivot table, or an epidemic “Epi” curve).
    9. Interpret the data collected and derive inferences regarding any relevant factors, such as ethnicity, geographical impact, and environmental factors on the population's health.

    Cite at least 2 sources used for your assignment in APA format.

    Format your assignment according to APA guidelines to include a brief introduction and clear conclusion.

    Submit your assignment as ONE document in Microsoft Word (not a PDF).

    Resources

    – [Center for Writing Excellence](https://multimedia.phoenix.edu/cms/cwe/)
    – [Reference and Citation Generator](https://multimedia.phoenix.edu/cms/citation)
    – [Grammar Assistance](https://multimedia.phoenix.edu/cms/202010168/)

    Copyright 2023 by University of Phoenix. All rights reserved.

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    ### Details & Information

    Assessment due date12/2/25, 12:59 AM (CST)

    Goals & standards
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    #### Grading

    Maximum points40 points

    #### Description

    This assessment measures your ability to develop data collection and information organization and retrieval plans.

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    **## HEalthinformationManagementUsingandUnderstandingpublichealthdatapaper.html**

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    ## Activity Stream

    FilterShow All

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    ### Upcoming

    –

    Nov 20, 2025
    11:27 PM

    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 1 Summative Assessment: Health Information Management Using and Understanding Public Health Data Paper

    Due Date: 12/2/25, 12:59 AM (CST)

    –

    Nov 20, 2025
    11:27 PM

    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 1 – Course Capstone Project – Preparation and Statement of Need

    Due Date: 12/2/25, 12:59 AM (CST)

    –

    Nov 20, 2025
    11:27 PM

    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 1 Discussion – Data Collection (initial response due Saturday)

    Due Date: 12/2/25, 12:59 AM (CST)

    –

    Nov 20, 2025
    11:27 PM

    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 2 – Course Capstone Project – Annotated Bibliography

    Due Date: 12/9/25, 12:59 AM (CST)

    –

    Nov 20, 2025
    11:27 PM

    [MPH/530: Epidemiology Concepts And Public Health Diseases](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)Due: Wk 2 Discussion – Disease Prevention [due Thurs]

    Due Date: 12/9/25, 12:59 AM (CST)

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    ## MPH/530: Epidemiology Concepts And Public Health Diseases

    OpenCourse status Open

    – [Content](https://vle.phoenix.edu/ultra/courses/_547585_1/outline)
    – [Calendar](https://vle.phoenix.edu/ultra/courses/_547585_1/calendar)
    – [Announcements3](https://vle.phoenix.edu/ultra/courses/_547585_1/announcements)
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    [**Course Content**](https://vle.phoenix.edu/ultra/courses/_547585_1/groups/enrollments)

    #### [Using AI at UOPX](https://ultra.content.blackboardcdn.com/ultra/uiv4000.1.0-rel.32_1e7dd2d#)

    Visit the Center for AI Resources to explore tools, tips, and examples to help you thrive in an AI-powered world. Check back regularly for updates.

    #### [Course Questions](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/discussion/_52313045_1?view=discussions&courseId=_547585_1)

    Have a question about this course? Ask here! Your faculty member will monitor this area. If you know the answer, feel free to help your peers by posting a reply.

    #### Welcome to the Course and Getting Started

    Start with this folder to meet your faculty member and peers. Review the course guide, syllabus, and grading rubrics for an overview of this course.

    #### Wk 1 – Community and Population Health Data

    [Wk 1 Overview](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/edit/document/_52313060_1?courseId=_547585_1&view=content&state=view) [Wk 1 Discussion – Data Collection (initial response due Saturday)](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/discussion/_52313047_1?view=discussions&courseId=_547585_1)

    Due date: 12/2/25, 12:59 AM (CST)

    Post a total of 3 substantive responses over 2 separate days for full participation. This includes your initial post and 2 replies to classmates or your faculty member. Due Saturday because of Thanksgiving To prepare for this week’s discussions, begin by selecting and reading 1 article from the University Library. Then, respond to the following questions in a minimum of 175 words: What data collection method(s) did the researcher use? Why did they choose this method(s)? Were they appropriate? Why or why not? Were the methods effective or ineffective for gathering data and proposing interventions for the targeted community? Why or why not? What rationale, examples, and comparisons would you use to defend your perspectives? What other …

    [Wk 1 Summative Assessment: Health Information Management Using and Understanding Public Health Data Paper](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/assessment/_52313062_1/overview?courseId=_547585_1)

    Due date: 12/2/25, 12:59 AM (CST)

    This assessment measures your ability to develop data collection and information organization and retrieval plans.

    [Wk 1 – Course Capstone Project – Preparation and Statement of Need](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/assessment/_52313063_1/overview?courseId=_547585_1)

    Due date: 12/2/25, 12:59 AM (CST)

    Wk 1 Learning Activities

    [Syringe Exchange Programs! A great harm reduction tool (video by Bob)](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/edit/document/_52322287_1?courseId=_547585_1&view=content&state=view)

    No more content items to load

    #### Wk 2 – Disease Prevention and Program Implementation

    #### Wk 3 – Social Justice and Health Equity in Epidemiology

    #### Wk 4 – Examining Epidemiology Research Design

    #### Wk 5 – Evaluating Epidemiology Research Methods

    #### Wk 6 – Applying Epidemiology Research Methods

    No more content items to load

    Press Enter or Space to start.

    ### Course Faculty

    – ![Bob Vella](https://www.sweetstudy.com/questions/health-information-20951863)

    Bob Vella

    Instructor

    [**Details & Actions**](https://ultra.content.blackboardcdn.com/ultra/uiv4000.1.0-rel.32_1e7dd2d#)

    –

    Roster

    View everyone in your course

    –

    Course Description

    View the course description

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    Class Collaborate

    Join session

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    Tools & Resources

    View course & institution tools

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    MPH/530: Epidemiology Concepts And Public Health Diseases

    Menu

    ## Wk 1 Summative Assessment: Health Information Management Using and Understanding Public Health Data Paper

    ### Assignment Instructions

    Research data from a provided case study to understand how it impacts the population.

    Write a 700- to 1,400-word paper in which you will include the following:

    1. Select 1 of the following case studies. Then, conduct additional research to gain an understanding of the chosen public health issue.
    2. [Notes from the Field: Multistate Outbreak of Escherichia coli O157:H7 Infections Linked to a National Fast-Food Chain — United States, 2022](https://www.cdc.gov/mmwr/volumes/72/wr/mm7226a6.htm)
    3. [Notes from the Field: Multistate, Multiserotype Outbreak of Salmonella Infections Linked to Cashew Brie — United States, 2021](https://www.cdc.gov/mmwr/volumes/72/wr/mm7221a4.htm)
    4. [Multistate Outbreak of Salmonella Thompson Infections Linked to Seafood Exposure — United States, 2021](https://www.cdc.gov/mmwr/volumes/72/wr/mm7219a2.htm)
    5. [Outbreaks Associated with Untreated Recreational Water — California, Maine, and Minnesota, 2018–2019](https://www.cdc.gov/mmwr/volumes/69/wr/mm6925a3.htm)
    6. [Notes From the Field: Campylobacteriosis Outbreak Associated with Consumption of Raw Water — Montana, 2022](https://www.cdc.gov/mmwr/volumes/72/wr/mm7215a6.htm)
    7. Identify data from within the selected case study. Summarize the demographic and clinical information of the data.
    8. Enter data into an Excel file and produce a graphical visual display of the data (e.g., chart or graph of demographic data, histogram, pivot table, or an epidemic “Epi” curve).
    9. Interpret the data collected and derive inferences regarding any relevant factors, such as ethnicity, geographical impact, and environmental factors on the population's health.

    Cite at least 2 sources used for your assignment in APA format.

    Format your assignment according to APA guidelines to include a brief introduction and clear conclusion.

    Submit your assignment as ONE document in Microsoft Word (not a PDF).

    Resources

    – [Center for Writing Excellence](https://multimedia.phoenix.edu/cms/cwe/)
    – [Reference and Citation Generator](https://multimedia.phoenix.edu/cms/citation)
    – [Grammar Assistance](https://multimedia.phoenix.edu/cms/202010168/)

    Copyright 2023 by University of Phoenix. All rights reserved.

    ### Submission

    Text Editor

    Word count: 0

    ### Details & Information

    Assessment due date12/2/25, 12:59 AM (CST)

    Goals & standards
    [Aligned with 1 goal](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/assessment/_52313062_1/overview/attempt/create/goalAlignment?courseId=_547585_1)

    AttemptsUnlimited

    Grading rubric
    [This item is graded with a rubric](https://vle.phoenix.edu/ultra/courses/_547585_1/outline/assessment/_52313062_1/overview/attempt/create/rubric-view?columnId=_11422305_1&courseId=_547585_1)

    Originality ReportSafeAssign enabled

    #### Grading

    Maximum points40 points

    #### Description

    This assessment measures your ability to develop data collection and information organization and retrieval plans.

    Save and CloseSubmit

    ×

    Help for current page

  • [Solved] biology

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    In-depth study guidance and analytical solution covering biology.

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  • [Solved] Biology

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  • [Solved] Genetic

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    Answer all questions – ## Genetic.docx **## Genetic.docx** _BIOL-2416 Baggett – Unit 04 – Study Tips_ ## Study Suggestions for Exam #4 over Chapters 14-16 & 18 ### General Exam Format and Information **What to bring:** knowledge and understanding, your signed 4×6 notecard, at least 2 sharpened penc…

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    Answer all questions

    – ## Genetic.docx

    **## Genetic.docx**

    _BIOL-2416 Baggett – Unit 04 – Study Tips_

    ## Study Suggestions for Exam #4 over Chapters 14-16 & 18

    ### General Exam Format and Information

    **What to bring:** knowledge and understanding, your signed 4×6 notecard, at least 2 sharpened pencils and a calculator if you have one (can borrow from me if needed during exam, but no phones allowed)

    **The exam will be administered from 6:00 p.m. to 8:00 p.m.** **No need to return after the exam this time**.

    **Question breakdown** (test total is 100 points)

    ·
    **40 regular multiple-choice questions** (2 pts each x 40 = 80 pts)

    · 4 chapters is ~8-12 questions per chapter for Ch. 14, 15, 16 and 18

    · you should go back and review the cell cycle (Ch. 2), chromosome mapping (Ch.5), chromosome structure and variation (Ch.6) and bacterial plasmids, transformation and immune-like defense (Ch. 7) as you study this unit since must of this content builds on topics from those chapters.

    · 2 or 3 of the multiple-choice questions will be from Genetics in Practice openers

    ·
    **3 short essay questions** (5-8 pts each)
    **plus 1 bonus short essay question** –
    ***For this exam, I have noted the concept most likely to show up in the short essay questions for you (see chapter headings)**

    ### Topics and Key Terms by Chapter –

    #### What to Study for Chapter 14 – _expect a short essay on combined: #5, #6 and #10_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Terms from chapter to review (in addition to those directly mentioned in specific topics below):
    **recombinant DNA, transgenic organism, nuclease, agarose, methylene blue, ethidium bromide (EtBr), SYBR Green, anneal / annealing, primer, antibiotic, antibiotic resistance**

    3. Describe the normal cellular origin and role of
    **restriction enzymes**, what types of sequences they recognize, how they cut DNA and what scientists use them for in the laboratory

    3.1. Distinguish between the type of cut that makes a cohesive (sticky) end versus a
    **blunt end**.

    3.2. Identify which restriction enzymes will produce larger fragments (cut less often) based on length of the
    **recognition sequence.**

    _**You will NOT be tested on the engineered nuclease section (no questions on this)._

    4. For
    **CRISPR-Cas** systems:

    4.1. Describe the normal cellular origin and role of CRISPR-Cas systems, how they know which sequences to recognize (
    **crRNA**), which part has nuclease activity to cut the DNA

    4.2. Explain how scientists have engineered this system to cut and modify any specific sequence they desire, including what
    **sgRNA** is and how
    **Non-Homologous End-Joining (NHEJ)** and
    **Homology Directed Repair** (
    **HDR**) enzymes are involved.

    5. Explain what
    **gel electrophoresis** is, what creates the gel matrix for DNA gels and how we use it to separate and visualize DNA.

    6. Describe the three steps of
    **Polymerase Chain Reaction (PCR)**, including why they are repeated, how primers are involved, where they bind (why just
    _outside_ the region you want to amplify?) & the key role of
    **_Taq_ polymerase.**

    7. Define “
    **gene cloning**” and describe the 3 requirements for any
    **cloning vector** to work

    7.1. Identify key characteristics of
    **plasmid vectors**, the basic
    **transformation** process and the two common hosts scientists use by the scientific names (which specific bacteria and yeast from Ch. 1)

    7.2. Describe the unique characteristics of
    **expression vectors** and how scientists obtain eukaryotic DNA that can be used with them (see next item)

    8. Describe how a
    **DNA library** is made, stored and the differences between making and using a
    **genomic library** versus a
    **cDNA library**

    9. Describe how
    **Sanger sequencing** (
    **dideoxy sequencing**) works and what is special about dideoxy nucleotides (
    **ddNTPs**) that allows them to be used in this method.

    10. Describe
    **microsatellites**(
    **short tandem repeats, STRs**) in DNA and explain how they are used in DNA fingerprinting or profiling to identify DNA samples.
    _Be able to relate this to #5 and #6 in a complete crime scene analysis procedure._

    11. Define or recognize examples of
    **forward genetics** versus
    **reverse genetics** methodologies.

    #### What to Study for Chapter 15 – _likely no short essay on this chapter since Ch. 18 has two_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Differentiate between
    **genomics** and
    **proteomics**.

    2.1. Describe the differences between the
    **Human Genome Project (HGP)** and the
    **Human Proteome Project (HPP)** in both their purpose and who they are examining humans (HGP focused on humans as whole organisms, while HPP is looking at different cell types
    _within_ humans)

    2.2. Define
    **structural genomics** and
    **functional** genomics in relation to these two terms.

    3. Describe the approach to sequencing a complex genome that ended up working for the HGP, including the role of
    **genetic linkage mapping, physical mapping, map-based sequencing** and whole genome
    **shotgun sequencing.**

    _**You will NOT be tested on NextGen or newer sequencing methods (no questions on this)._

    4. Describe what a
    **Single Nucleotide Polymorphism (SNP)** is and why a single SNP does not provide much information about a person or group of people, but a
    **haplotype** can.

    4.1. Explain how a
    **Genome-Wide Association Study (GWAS)** uses genome sequence and SNP information to being to analyze possible causes of a genetic condition, including the limitations of these studies.

    5. Identify the interdisciplinary fields involved in
    **bioinformatics** and why.

    6. Describe what
    **metagenomics** is and that it primarily focuses on microbial communities (and why helpful there)

    _**You will NOT be tested on using BLAST to analyze homologues or orthologs or RNA-seq (no questions on this)._

    7. Describe what a
    **microarray** is and how it is used by scientists to analyze gene expression differences between cell types or groups of individuals to identify genes involved in a process (such as cancer patients or developmental changes)

    8. For
    **comparative genomics**, review and be able to discuss basic features of prokaryotic versus eukaryotic genomes (general size of genome, # of genes and gene “density”) and the impact of
    **transposons** in repetitive DNA in eukaryotic genomes.

    #### What to Study for Chapter 16 – _expect a short essay on combined #3, #4 and #6_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Terms from chapter to review (in addition to those directly mentioned in specific topics below):
    **cell cycle checkpoints, contact inhibition, density dependent inhibition, metastasis / metastasize / metastatic, benign, malignant**

    3. Describe the primary characteristics of
    **cancer** (differentiating it from normal cells) and explain why it is a
    _group_ of genetic diseases, not just one.

    4. Explain why cancer is not typically inherited and why most non-inherited cancer arises later in life rather than in young people

    5. Identify the environmental factor that contributes
    **_most_** to the development of cancer (
    **tobacco**) as well as the relative
    **_overall impact_** of environmental factors on cancer development (
    **around 40%** of cancer cases).

    6. Describe how cancer develops, including what
    **clonal evolution** means and the genetic changes involved in developing a
    **tumor**, how it becomes
    **malignant** and how
    **metastasis** occurs.

    7. Identify what the proteins encoded by
    **proto-oncogenes** normally do in the cell and why mutations in these genes are often dominant alleles (why are most
    **oncogenes** dominant?)

    7.1. Recognize the proto-oncogene example
    **_ras_** has an oncogene mutant version present in 1/3 of all tumors

    8. Identify what roles the proteins encoded by
    **tumor suppressor genes** normally play in the cell and why mutations in these genes are often recessive

    8.1. Recognize the key examples of tumor suppressor genes discussed and their normal functions:
    **p53**,
    **RB**,
    **BRCA1** (and not listed in chart, but there is also a BRCA2 with similar function)

    8.2. Explain what
    **haploinsuffiency** is and how it might affect a heterozygote who inherited one knock-out allele for a tumor suppressor gene

    8.3. Describe how inheriting one knock-out allele for a tumor suppressor gene might lead to increased development of cancer even though the allele is recessive (i.e. explain
    **loss of heterozygosity** cause & effect)

    _**You will NOT be tested on cyclins and cdks, though I may mention them in a question, so recognize that they are involved in checkpoints and forward/stop signals so you aren’t confused by the mention._

    9. Describe how
    **retinoblastoma (Rb) cancer** develops, including which cell cycle checkpoint fails (G1-to-S) and what the
    **2-hit hypothesis** explains about inherited versus sporadic (non-inherited) retinoblastoma tumors (which are almost always
    **unilateral** and which are often
    **bilateral** and why).

    10. Recognize and identify the role of secondary mutations in genes for
    **telomerase**,
    **angiogenesis** and
    **cytoskeletal** and
    **ECM structure** as well as
    **aneuploidy** and large chromosomal mutations on tumor development and cancer progression.

    11. Identify the 2 key ways that
    **viruses**, especially
    **retroviruses** like HPV, can cause cancer after infection.

    #### What to Study for Chapter 18 – _expect a short essay on #3_ _or #5 AND also on #7, including 7.1_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Terms from chapter to review (in addition to those directly mentioned in specific topics below):
    **evolution, gene pool, genotypic frequency, allelic frequency, pre-zygotic, post-zygotic, speciation**

    3. Calculate genotypic and allelic frequencies for an autosomal locus with two alleles given population information (total number of individuals and number of individuals with each phenotype).

    4. Describe the
    **Hardy-Weinberg law** (i.e. equations) and the conditions required for
    **genetic** **equilibrium** to exist in a population.

    5. Apply the predictions of the Hardy-Weinberg law to calculate genotypic and allelic frequencies for populations in HW equilibrium given one piece of information (such as q2)

    6. Describe the forces that drive evolution (
    **genetic drift**,
    **nonrandom mating**,
    **mutation**,
    **migration**, and
    **natural selection**) and their effects on allelic frequencies of a population.

    6.1. For genetic drift, also explain the causes of genetic drift (
    **sampling error**, randomness), what factors influence the magnitude of drift and what
    **bottleneck** **effect** and
    **founder effect** are.

    6.2. For natural selection, explain how evolutionary change of this type differs from the others

    6.3. Identify which forces increase diversity and which decrease diversity WITHIN a population

    6.4. Identify which forces increase diversity and which decrease diversity BETWEEN SEPARATE populations

    7. Recite the
    **biological species** **definition** and describe its
    **limitations**.

    7.1. Be ready to define
    **Reproductive Isolating Mechanism (RIM)** and describe at least two examples.

    8. Explain the difference between
    **allopatric** and
    **sympatric speciation**

    8.1. Identify the forces that drive evolution in each type of speciation.

    9. Explain how
    **phylogenetic trees** are used to show evolutionary relationships and the difference between
    **anagenesis** and
    **cladogenesis** in evolutionary relationships and appearance on the tree.

    10. Explain why rates of
    **nucleotide substitutions** vary among different parts of the genome and within a gene.

    11. Define the
    **molecular clock**, including the key assumption it makes and how scientists apply it to evolutionary studies.

    _Page 2 of 2_

    **## Genetic.docx**

    _BIOL-2416 Baggett – Unit 04 – Study Tips_

    ## Study Suggestions for Exam #4 over Chapters 14-16 & 18

    ### General Exam Format and Information

    **What to bring:** knowledge and understanding, your signed 4×6 notecard, at least 2 sharpened pencils and a calculator if you have one (can borrow from me if needed during exam, but no phones allowed)

    **The exam will be administered from 6:00 p.m. to 8:00 p.m.** **No need to return after the exam this time**.

    **Question breakdown** (test total is 100 points)

    ·
    **40 regular multiple-choice questions** (2 pts each x 40 = 80 pts)

    · 4 chapters is ~8-12 questions per chapter for Ch. 14, 15, 16 and 18

    · you should go back and review the cell cycle (Ch. 2), chromosome mapping (Ch.5), chromosome structure and variation (Ch.6) and bacterial plasmids, transformation and immune-like defense (Ch. 7) as you study this unit since must of this content builds on topics from those chapters.

    · 2 or 3 of the multiple-choice questions will be from Genetics in Practice openers

    ·
    **3 short essay questions** (5-8 pts each)
    **plus 1 bonus short essay question** –
    ***For this exam, I have noted the concept most likely to show up in the short essay questions for you (see chapter headings)**

    ### Topics and Key Terms by Chapter –

    #### What to Study for Chapter 14 – _expect a short essay on combined: #5, #6 and #10_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Terms from chapter to review (in addition to those directly mentioned in specific topics below):
    **recombinant DNA, transgenic organism, nuclease, agarose, methylene blue, ethidium bromide (EtBr), SYBR Green, anneal / annealing, primer, antibiotic, antibiotic resistance**

    3. Describe the normal cellular origin and role of
    **restriction enzymes**, what types of sequences they recognize, how they cut DNA and what scientists use them for in the laboratory

    3.1. Distinguish between the type of cut that makes a cohesive (sticky) end versus a
    **blunt end**.

    3.2. Identify which restriction enzymes will produce larger fragments (cut less often) based on length of the
    **recognition sequence.**

    _**You will NOT be tested on the engineered nuclease section (no questions on this)._

    4. For
    **CRISPR-Cas** systems:

    4.1. Describe the normal cellular origin and role of CRISPR-Cas systems, how they know which sequences to recognize (
    **crRNA**), which part has nuclease activity to cut the DNA

    4.2. Explain how scientists have engineered this system to cut and modify any specific sequence they desire, including what
    **sgRNA** is and how
    **Non-Homologous End-Joining (NHEJ)** and
    **Homology Directed Repair** (
    **HDR**) enzymes are involved.

    5. Explain what
    **gel electrophoresis** is, what creates the gel matrix for DNA gels and how we use it to separate and visualize DNA.

    6. Describe the three steps of
    **Polymerase Chain Reaction (PCR)**, including why they are repeated, how primers are involved, where they bind (why just
    _outside_ the region you want to amplify?) & the key role of
    **_Taq_ polymerase.**

    7. Define “
    **gene cloning**” and describe the 3 requirements for any
    **cloning vector** to work

    7.1. Identify key characteristics of
    **plasmid vectors**, the basic
    **transformation** process and the two common hosts scientists use by the scientific names (which specific bacteria and yeast from Ch. 1)

    7.2. Describe the unique characteristics of
    **expression vectors** and how scientists obtain eukaryotic DNA that can be used with them (see next item)

    8. Describe how a
    **DNA library** is made, stored and the differences between making and using a
    **genomic library** versus a
    **cDNA library**

    9. Describe how
    **Sanger sequencing** (
    **dideoxy sequencing**) works and what is special about dideoxy nucleotides (
    **ddNTPs**) that allows them to be used in this method.

    10. Describe
    **microsatellites**(
    **short tandem repeats, STRs**) in DNA and explain how they are used in DNA fingerprinting or profiling to identify DNA samples.
    _Be able to relate this to #5 and #6 in a complete crime scene analysis procedure._

    11. Define or recognize examples of
    **forward genetics** versus
    **reverse genetics** methodologies.

    #### What to Study for Chapter 15 – _likely no short essay on this chapter since Ch. 18 has two_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Differentiate between
    **genomics** and
    **proteomics**.

    2.1. Describe the differences between the
    **Human Genome Project (HGP)** and the
    **Human Proteome Project (HPP)** in both their purpose and who they are examining humans (HGP focused on humans as whole organisms, while HPP is looking at different cell types
    _within_ humans)

    2.2. Define
    **structural genomics** and
    **functional** genomics in relation to these two terms.

    3. Describe the approach to sequencing a complex genome that ended up working for the HGP, including the role of
    **genetic linkage mapping, physical mapping, map-based sequencing** and whole genome
    **shotgun sequencing.**

    _**You will NOT be tested on NextGen or newer sequencing methods (no questions on this)._

    4. Describe what a
    **Single Nucleotide Polymorphism (SNP)** is and why a single SNP does not provide much information about a person or group of people, but a
    **haplotype** can.

    4.1. Explain how a
    **Genome-Wide Association Study (GWAS)** uses genome sequence and SNP information to being to analyze possible causes of a genetic condition, including the limitations of these studies.

    5. Identify the interdisciplinary fields involved in
    **bioinformatics** and why.

    6. Describe what
    **metagenomics** is and that it primarily focuses on microbial communities (and why helpful there)

    _**You will NOT be tested on using BLAST to analyze homologues or orthologs or RNA-seq (no questions on this)._

    7. Describe what a
    **microarray** is and how it is used by scientists to analyze gene expression differences between cell types or groups of individuals to identify genes involved in a process (such as cancer patients or developmental changes)

    8. For
    **comparative genomics**, review and be able to discuss basic features of prokaryotic versus eukaryotic genomes (general size of genome, # of genes and gene “density”) and the impact of
    **transposons** in repetitive DNA in eukaryotic genomes.

    #### What to Study for Chapter 16 – _expect a short essay on combined #3, #4 and #6_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Terms from chapter to review (in addition to those directly mentioned in specific topics below):
    **cell cycle checkpoints, contact inhibition, density dependent inhibition, metastasis / metastasize / metastatic, benign, malignant**

    3. Describe the primary characteristics of
    **cancer** (differentiating it from normal cells) and explain why it is a
    _group_ of genetic diseases, not just one.

    4. Explain why cancer is not typically inherited and why most non-inherited cancer arises later in life rather than in young people

    5. Identify the environmental factor that contributes
    **_most_** to the development of cancer (
    **tobacco**) as well as the relative
    **_overall impact_** of environmental factors on cancer development (
    **around 40%** of cancer cases).

    6. Describe how cancer develops, including what
    **clonal evolution** means and the genetic changes involved in developing a
    **tumor**, how it becomes
    **malignant** and how
    **metastasis** occurs.

    7. Identify what the proteins encoded by
    **proto-oncogenes** normally do in the cell and why mutations in these genes are often dominant alleles (why are most
    **oncogenes** dominant?)

    7.1. Recognize the proto-oncogene example
    **_ras_** has an oncogene mutant version present in 1/3 of all tumors

    8. Identify what roles the proteins encoded by
    **tumor suppressor genes** normally play in the cell and why mutations in these genes are often recessive

    8.1. Recognize the key examples of tumor suppressor genes discussed and their normal functions:
    **p53**,
    **RB**,
    **BRCA1** (and not listed in chart, but there is also a BRCA2 with similar function)

    8.2. Explain what
    **haploinsuffiency** is and how it might affect a heterozygote who inherited one knock-out allele for a tumor suppressor gene

    8.3. Describe how inheriting one knock-out allele for a tumor suppressor gene might lead to increased development of cancer even though the allele is recessive (i.e. explain
    **loss of heterozygosity** cause & effect)

    _**You will NOT be tested on cyclins and cdks, though I may mention them in a question, so recognize that they are involved in checkpoints and forward/stop signals so you aren’t confused by the mention._

    9. Describe how
    **retinoblastoma (Rb) cancer** develops, including which cell cycle checkpoint fails (G1-to-S) and what the
    **2-hit hypothesis** explains about inherited versus sporadic (non-inherited) retinoblastoma tumors (which are almost always
    **unilateral** and which are often
    **bilateral** and why).

    10. Recognize and identify the role of secondary mutations in genes for
    **telomerase**,
    **angiogenesis** and
    **cytoskeletal** and
    **ECM structure** as well as
    **aneuploidy** and large chromosomal mutations on tumor development and cancer progression.

    11. Identify the 2 key ways that
    **viruses**, especially
    **retroviruses** like HPV, can cause cancer after infection.

    #### What to Study for Chapter 18 – _expect a short essay on #3_ _or #5 AND also on #7, including 7.1_

    1. Genetics in Practice – make sure you know the answers to the questions I asked in the chapter PowerPoint.

    2. Terms from chapter to review (in addition to those directly mentioned in specific topics below):
    **evolution, gene pool, genotypic frequency, allelic frequency, pre-zygotic, post-zygotic, speciation**

    3. Calculate genotypic and allelic frequencies for an autosomal locus with two alleles given population information (total number of individuals and number of individuals with each phenotype).

    4. Describe the
    **Hardy-Weinberg law** (i.e. equations) and the conditions required for
    **genetic** **equilibrium** to exist in a population.

    5. Apply the predictions of the Hardy-Weinberg law to calculate genotypic and allelic frequencies for populations in HW equilibrium given one piece of information (such as q2)

    6. Describe the forces that drive evolution (
    **genetic drift**,
    **nonrandom mating**,
    **mutation**,
    **migration**, and
    **natural selection**) and their effects on allelic frequencies of a population.

    6.1. For genetic drift, also explain the causes of genetic drift (
    **sampling error**, randomness), what factors influence the magnitude of drift and what
    **bottleneck** **effect** and
    **founder effect** are.

    6.2. For natural selection, explain how evolutionary change of this type differs from the others

    6.3. Identify which forces increase diversity and which decrease diversity WITHIN a population

    6.4. Identify which forces increase diversity and which decrease diversity BETWEEN SEPARATE populations

    7. Recite the
    **biological species** **definition** and describe its
    **limitations**.

    7.1. Be ready to define
    **Reproductive Isolating Mechanism (RIM)** and describe at least two examples.

    8. Explain the difference between
    **allopatric** and
    **sympatric speciation**

    8.1. Identify the forces that drive evolution in each type of speciation.

    9. Explain how
    **phylogenetic trees** are used to show evolutionary relationships and the difference between
    **anagenesis** and
    **cladogenesis** in evolutionary relationships and appearance on the tree.

    10. Explain why rates of
    **nucleotide substitutions** vary among different parts of the genome and within a gene.

    11. Define the
    **molecular clock**, including the key assumption it makes and how scientists apply it to evolutionary studies.

    _Page 2 of 2_

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    – ## researchasituationwhereahealthcareorganizationinyourfieldofalliedhealthwassanctionedbytheaccreditingbody.docx

    **## researchasituationwhereahealthcareorganizationinyourfieldofalliedhealthwassanctionedbytheaccreditingbody.docx**

    Perform an internet search to identify and research a situation where a health care organization or individual provider in your field of allied health was sanctioned by the accrediting body (e.g., The Joint Commission) and another regulatory body for violating one or more of the accrediting body's workplace safety, risk management, and/or quality care requirements.

    Taking on the role of chief safety or risk management officer in the organization or provider's office who now must deliver an account of the chosen incident to the board of directors, develop a slide presentation containing a title slide, 12-15 slides of content, and a reference slide.

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    · A summary of the applicable MIPPA approved accrediting body, other regulatory standards (e.g., local, state), or licensing/certification standards that apply to the incident.

    · A discussion of the mistakes and/or oversights made by the health care organization or individual provider that did or may have led to the incident that occurred, and an account of the preventive steps that could have or should have been taken to avoid them.

    · A proposal outlining specific education or training the organization or provider will employ to ensure this type of incident does not occur in the future. Include concepts related to continuous quality improvement in your recommendations.

    · An inclusion of a CQI tool to be used to implement the training or education program being proposed.

    To create your presentation, use Microsoft PowerPoint or another program such as Prezi. Speaker's notes must be included for each slide. Refer to the resource, "Creating Effective PowerPoint Presentations," located in the Student Success Center, for additional guidance on completing this assignment in the appropriate style.

    Provide a minimum of three qualified resources from the readings or the GCU Library in order to complete this assignment successfully.

    While APA format is not required for the body of this assignment, solid academic writing is expected, and in-text citations and references should be presented using APA documentation guidelines, which can be found in the APA Style Guide, located in the Student Success Center.

    This assignment uses a rubric. Please review the rubric prior to beginning the assignment to become familiar with the expectations for successful completion.

    You are required to submit this assignment to LopesWrite. A link to the LopesWrite technical support articles is located in Course Resources if you need assistance.

    **## researchasituationwhereahealthcareorganizationinyourfieldofalliedhealthwassanctionedbytheaccreditingbody.docx**

    Perform an internet search to identify and research a situation where a health care organization or individual provider in your field of allied health was sanctioned by the accrediting body (e.g., The Joint Commission) and another regulatory body for violating one or more of the accrediting body's workplace safety, risk management, and/or quality care requirements.

    Taking on the role of chief safety or risk management officer in the organization or provider's office who now must deliver an account of the chosen incident to the board of directors, develop a slide presentation containing a title slide, 12-15 slides of content, and a reference slide.

    Your presentation must incorporate the following:

    · A brief summary of the incident, including a description as well as the outcome.

    · A summary of the applicable MIPPA approved accrediting body, other regulatory standards (e.g., local, state), or licensing/certification standards that apply to the incident.

    · A discussion of the mistakes and/or oversights made by the health care organization or individual provider that did or may have led to the incident that occurred, and an account of the preventive steps that could have or should have been taken to avoid them.

    · A proposal outlining specific education or training the organization or provider will employ to ensure this type of incident does not occur in the future. Include concepts related to continuous quality improvement in your recommendations.

    · An inclusion of a CQI tool to be used to implement the training or education program being proposed.

    To create your presentation, use Microsoft PowerPoint or another program such as Prezi. Speaker's notes must be included for each slide. Refer to the resource, "Creating Effective PowerPoint Presentations," located in the Student Success Center, for additional guidance on completing this assignment in the appropriate style.

    Provide a minimum of three qualified resources from the readings or the GCU Library in order to complete this assignment successfully.

    While APA format is not required for the body of this assignment, solid academic writing is expected, and in-text citations and references should be presented using APA documentation guidelines, which can be found in the APA Style Guide, located in the Student Success Center.

    This assignment uses a rubric. Please review the rubric prior to beginning the assignment to become familiar with the expectations for successful completion.

    You are required to submit this assignment to LopesWrite. A link to the LopesWrite technical support articles is located in Course Resources if you need assistance.

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    Now that you have selected one measurable outcome from your logic model (Week 2), this week we will determine how the evaluation will be conducted and who will be included in it. For this discussion, address the following: **1. Outcome Being Evaluated** State one short-term or intermediate outcome …

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    Assignment Prompt & Instructions

    Now that you have selected one measurable outcome from your logic model (Week 2), this week we will determine how the evaluation will be conducted and who will be included in it.
    For this discussion, address the following:
    **1. Outcome Being Evaluated**
    State one short-term or intermediate outcome from your logic model.
    Restate it clearly as an outcome, not an activity or goal.
    **2. Evaluation Design**
    What would be the best design to evaluate the outcome at your agency? Most program evaluations use a self-controlled pre/post design (comparing each participant’s outcome measure at intake and again at post-treatment or discharge), but you should confirm whether this design fits your program.
    – Explain why the design you chose is feasible in your agency.
    – _Do not create a new control group or add groups that do not exist in practice._
    **3. Limitations of the Design**
    Briefly discuss one limitation of using this design.
    Choose only one that realistically applies to your setting, such as:
    – Maturation
    – Regression to the mean
    – History effects
    – Selection bias
    Explain why the chosen limitation matters for _your_ outcome, in everyday language.
    **4. Sampling Strategy**
    Describe who will be included in the evaluation.
    Explain why this sampling approach is appropriate for your program.
    **Reminder:** Your response must reference your specific program AND the specific outcome you identified. Only one citation required this week.
    **Post your initial response to the discussion question early in the learning week (no later than Wednesday) and then respond to a minimum of two other posts no later than Sunday.**
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